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Croda International Plc
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Avanti Polar
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Cambridge Isotope Laboratories
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Croda International Plc
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Avanti Inc
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Croda International Plc
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Croda International Plc
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Croda International Plc
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Croda International Plc
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Avanti Polar
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Croda International Plc
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Image Search Results
Journal: Scientific Reports
Article Title: Cerebrospinal fluid metabolomics, lipidomics and serine pathway dysfunction in myalgic encephalomyelitis/chronic fatigue syndroome (ME/CFS)
doi: 10.1038/s41598-025-91324-1
Figure Lengend Snippet: Hedges’ g for logarithmically transformed, normalized and standardized ME/CFS ( n = 59) vs. SC ( n = 35) by two-tailed unpaired t-test.
Article Snippet: EquiSPLASH ® LIPIDOMIX ® quantitative mass spec internal standard and 15:0–18:1-d7-PA,
Techniques: Transformation Assay
Journal: Science Advances
Article Title: Progressive alterations in amino acid and lipid metabolism correlate with peripheral neuropathy in Polg D257A mice
doi: 10.1126/sciadv.abj4077
Figure Lengend Snippet: ( A ) Schematic of major plasma lipids. ( B ) Concentration of plasma cholesterol in WT and Polg mice. Concentration of total ( C ) and individual ( D ) plasma fatty acids in WT and Polg mice. Abundance of plasma triacylglycerols ( E ) and diacylglycerols ( F ), phosphatidylethanolamine (PE) ( G ), and phosphatidylcholine (PC) ( H ) relative to internal standard per microliter of plasma in WT and Polg mice. Concentration of plasma dihydroceramide ( I ), ceramide ( J ), sphingomyelin ( K ), glucosylceramide ( L ), and lactosylceramide ( M ) in WT and Polg mice. Two-sided Student’s t test for each comparison with no adjustment for multiple comparisons. Data are means ± SEM of n = 7 to 8 animals per group. * P < 0.05, ** P < 0.01, and **** P < 0.0001.
Article Snippet: For targeted sphingolipid analysis, 50 μl of plasma was extracted with 500 μl of −20°C methanol, 400 μl of saline, and 100 μl of water spiked with deuterated internal standards [100 pmol of D7-sphingosine (Avanti, Croda International Plc, 860657), 20 pmol of D7-sphinganine (Avanti, Croda International Plc, 860658), 2 pmol of D3-deoxysphinganine (Avanti, Croda International Plc, 860474), 200 pmol of C15 ceramide-d7 (d18:1-d7/15:0) (Avanti, Croda International Plc, 860681), 100 pmol of C13-dihydroceramide-d7 (d18:0-d7/13:0) (Avanti, Croda International Plc, 330726), 10 pmol of glucosylsphingosine (d18:1-d7) (Avanti, Croda International Plc, 860695), 100 pmol of
Techniques: Concentration Assay
Journal: Scientific reports
Article Title: Parallel Reaction Monitoring reveals structure-specific ceramide alterations in the zebrafish.
doi: 10.1038/s41598-019-56466-z
Figure Lengend Snippet: Figure 1. A Parallel Reaction Monitoring-based approach for ceramide quantification. (a) MS2 of d18:1/16:0 ceramide standard in positive ionisation mode. Fragment structures are based on published assignments43,44. (b) Schematic of Parallel Reaction Monitoring. (c) Example PRM chromatograms for C41:1 ceramide from 48 hpf zebrafish embryos, demonstrating presence of multiple LCB-acyl chain isomers with the same m/z. RT: retention time. (d) MS2 spectrum of C41:1 ceramide from 48 hpf zebrafish embryos, the four major LCB fragments are labelled. (d,e) m/z 200-m/z 350, the four major LCB fragments and additional LCB-derived minor fragments are labelled. Unlabelled minor fragments: m/z 224.2371 (d16:1 LCB), m/z 238.2525 (d17:1 LCB).
Article Snippet: Materials. d18:1/16:0 (860516), d18:1/17:0 (860517), d18:1-d7/15:0 (860681),
Techniques: Targeted Proteomics, Derivative Assay